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Non-invasive imaging of cancer cells expressing fluorescent proteins has allowed the real-time determination of efficacy of candidate antitumor and antimetastatic agents in mouse models.

The use of fluorescent proteins to differentially label cancer cells in the nucleus and cytoplasm can visualize the nuclear–cytoplasmic dynamics of cancer cells in vivo including: mitosis, apoptosis, cell-cycle position, and differential behavior of nucleus and cytoplasm that occurs during cancer-cell deformation and extravasation.

Fluorescent proteins generally have very high extinction coefficients ranging up to approximately =95 000.

In addition, fluorescent proteins have very high quantum yields up to 0.8.

The optics of the OV100 fluorescence imaging system have been specially developed for macroimaging as well as microimaging with high light-gathering capacity.

The objectives have high numerical aperture and long working distance.

High-resolution images are captured directly on a PC (Fujitsu Siemens, Munich, Germany).

The excitation light was produced with a simple blue-LED flashlight equipped with an excitation filter with a central peak of 470 nm (shown in the figure).

Another important feature of fluorescent proteins is the spectral distinction between many members of the family, enabling of multicolor fluorescent proteins to be used simultaneously for multifunctional in vivo imaging.

Spectral separation imaging is also very useful to distinguish different colors including autofluorescence.

The OV-100 has the lenses mounted on an automated turret with a high magnification range of × 1.6 to × 16 and a field of view ranging from 6.9 to 0.69 mm.

The optics and antireflective coatings ensure optimal imaging of multiplexed fluorescent reporters in small animals.

For example, the behavior of cancer stem cells labeled with GFP and non-stem cells labeled with red fluorescent protein (RFP) can be simultaneously compared in vivo.

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