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Villous CTs were isolated from term placentas and cultured as described previously (Pidoux et al., 2014).

Be Wo cells were obtained from American Type Culture Collection (Manassas, VA, USA) and cultured as described by manufacturer. Localized effects of c AMP mediated by distinct routes of protein kinase A.

Cell extracts were prepared as described previously (Pidoux et al., 2014).

Protein samples were resolved by SDS-PAGE and immunoblotted with antibodies (catalog numbers and supplier are indicated unless stated above) against PKA RIα (0.25 μg/ml; 4D7; BD Biosciences), PKA RIIα (0.25 μg/ml; 612243; BD Biosciences), PKA RIIβ (0.25 μg/ml; 610626; BD Biosciences); PKA Cα/β (0.25 μg/ml; 610980; BD Biosciences); AKAP18 (0.5 μg/ml; WH0009465M1; Sigma–Aldrich); GAPDH (1 μg/ml; G8795; Sigma–Aldrich). doi: 10.3389/fncel.2013.00211 Pub Med Abstract | Cross Ref Full Text | Google Scholar Schillace, R.

(Right, Lower) Human trophoblast stained at 24 and 72 h of culture for desmoplakin (magenta) and nuclei (DAPI, cyan). The c AMP signaling pathway is known to play a critical role in induction of CT and myoblast cell fusion reviewed in (Gerbaud and Pidoux, 2015).

For instance, in human placentation, h CG stimulates cytrophoblast fusion in an autocrine or paracrine fashion through binding to the LH/CG receptor (LH/CG-R), activating a specific adenylyl cyclase (AC) and the synthesis of intracellular c AMP as second messenger (Figure 2).

Furthermore, A-kinase anchoring proteins (AKAPs) provide through PKA anchoring a spatial regulation of the c AMP/PKA signaling by placing the kinase in the vicinity substrates. Reciprocal regulation of m RNA and protein for subunits of c AMP-dependent protein kinase (RI alpha and C alpha) by c AMP in a neoplastic B cell line (Reh).

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Due to their capacity to differentiate into syncytia allowing essential placental exchange between mother and child necessary for fetal growth, the CT plays an essential role during human pregnancy. Alternative splicing regulates the subcellular localization of A-kinase anchoring protein 18 isoforms. Whereas, PKA is known to be the main c AMP effector, other intracellular effectors exist such as exchange proteins activated by c AMP (Epac) and the cyclic nucleotide-gated ion channels (Walsh et al., 1968; Nakamura and Gold, 1987; de Rooij et al., 1998; Kawasaki et al., 1998) (Figure 2). The c AMP signaling pathway is one of the best-characterized signal transduction pathways and requires a high level of spatial and temporal regulation to convey the appropriate inputs. During human placentation, mononuclear cytotrophoblasts fuse to form multinucleated syncytia ensuring hormonal production and nutrient exchanges between the maternal and fetal circulation.

Syncytial formation is essential for the maintenance of pregnancy and for fetal growth.However, we have examined the functional role of AKAP-anchored signaling complexes human primary CTs in a recent report (Pidoux et al., 2014).



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